Molecular Characterization of High-Risk Human Papillomavirus in Women in Bobo-Dioulasso, Burkina Faso.

Ina Marie Angèle Traore,Germain Traore,Théodora Mahoukèdè Zohoncon,Charlemagne Ouedraogo,Florencia W Djigma,Yves Traore,Dorcas Obiri-Yeboah,Jacques Simpore,Moussa Bambara,Adama Dembele

doi:10.1155/2016/7092583

Abstract

High-risk human papillomavirus (HPV) is found in over 99% of cervical cancers. The aim of this study was to determine the prevalence of HPV in a population of women in Bobo-Dioulasso and to identify the high-risk types present in these women. From May to June, 2015, 181 women who came for consultation at the Souro Sanou University Hospital of Bobo-Dioulasso have been included in this study. Uterine endocervical swabs have been taken in these women. DNA obtained by extraction from the samples thus collected was used to determine the prevalence of high-risk human papillomavirus genotypes through real-time PCR. The age of the women ranged from 20 to 56 years with a mean of 35.3 ± 8.1 years. The prevalence of infection by high-risk HPV types was 25.4% (46/181). The most common high-risk HPV genotypes were HPV 39 (18.5%), HPV 52 (16.7%), HPV 18 (14.8%), and HPV 35 (13.0%). HPV 16 which is included in the HPV vaccines was not found in the population studied. This type of study which is the first one in Bobo-Dioulasso has showed a high prevalence of genotypes HPV 39, HPV 52, and HPV 35 which are not yet covered by a vaccine.

Highlights

The human papillomavirus (HPV) infection is one of the most common sexually transmitted infections in the world [1, 2]
When these lesions are not treated, some are likely to progress into cancer and HPV is found in 99.7% of cervical cancers [5]
In Burkina Faso, the study conducted by Zohoncon et al in 2013 based on two previous studies in Ouagadougou [9, 10] showed that the high-risk HPV genotypes which were the most common were HPV 35 and HPV 52 [11]

Summary

Introduction

The human papillomavirus (HPV) infection is one of the most common sexually transmitted infections in the world [1, 2]. The persistence of HPV infection with high oncogenetic risk is a condition for the occurrence of cervical precancerous and cancerous lesions [4]. While HPV 16 and HPV 18 genotypes are involved in approximately 70% of cancers of the cervix in the world [7], BioMed Research International the distribution of the other genotypes follows geographic variation. This difference in the distribution of genotypes was found in women who were living in different regions of the same country [8]. The aim of this study was to determine the prevalence of high-risk human papillomavirus in a population of women in Bobo-Dioulasso and to identify genotypes found in this city

Objectives

Methods

Results

Conclusion