Abstract
Research related to physiology and pathology of honey bees in particular Apis mellifera adansonii has attracted a lot of attention. The present study is aimed to determine honeybee (Apis mellifera adansonii) gut microbiome from Apiary in Olabisi Onabanjo University and Osoba Avenue Odo-Epo, Odogbolu Local Government. Twenty (20) honeybees workers (A. mellifera) were collected into a small vile containing sugar powder from the apiary located in OOU and Osoba Avenue at Odo-Epo during rainy season in July and transported to Zoology and Environmental Biology laboratory in OOU and kept in ice-cubes (-50C) till daybreak. Standard microbiological analysis for isolation of bacteria was used, adopting Clinical Laboratory Standard Institute procedures. The phylogenetic analyses based on the 16S rDNA gene were further used to characterize the organism in order to establish relationships among them. The results showed microbiota of the studied samples includes; Cedeca davisae, Cronobacter dublinensis, Enterobacter aerogenes, Kluyvera cryocrescens, Klebsiella oxytoca, Providencia vermicola, Salmonella enteric, Providencia alcalifaciens, Serratia nematodiphila, Pseudomonas plecoqlossicida, Klebsiella michiganensis, Serratia marcenscens, Pseudomonas aeruginosa, Escherichia coli, Aeromonas hydrophila and Enterobacter asburiae. Klebsiella spp. was more abundant and prominent in the digestive guts of honeybee workers both in OOU and Osoba Avenue, Odo Epo. The result of the percentage identity and closest accession of the isolates revealed that, Enterobacter aerogenes had the closest accession number and with highest percentage identity of (99%). The findings from this study showed that microbiota component communities of A. mellifera adansonii in OOU were composed of more Gram-negative bacteria than Gram-positive bacteria in Odo Epo.
Highlights
Research related to physiology and pathology of honey bees in particular Apis mellifera adansonii has attracted a lot of attention (Muli et al, 2014)
Bacteriological analysis along with molecular techniques based on 16S rRNA sequences precisely characterize insects gut bacterial flora
Sample Collection of Honey Bee: In order to study the cultivable honey bee gut bacteria, 20 honeybees of (A. mellifera) unknown age were collected each from the apiary located in OOU and Odo Epo
Summary
Research related to physiology and pathology of honey bees in particular Apis mellifera adansonii has attracted a lot of attention. The present study is aimed to determine honeybee (Apis mellifera adansonii) gut microbiome from Apiary in Olabisi Onabanjo University and Osoba Avenue Odo-Epo, Odogbolu Local Government. The findings from this study showed that microbiota component communities of A. mellifera adansonii in OOU were composed of more Gram-negative bacteria than Gram-positive bacteria in Odo Epo. Honey is a sweet food created by bees using nectar from flowers. Numerous causes of severe honey bee colony losses have been proposed, including pesticides toxicity, poor nutrition and genetic diversity This called for a deeper understanding of both symbiotic and pathogenic microbial interactions, as they relate to food storage and the pollination environment. The foregut is the honeybee's social/nutritional interface This portion of the alimentary tract is essentially an inflatable storage bag used to transport nectar from the flower to the hive, and selectively pass pollen into the midgut. Longitude of 3.9216oE respectively while Odo Epo has a latitude of 6.7888oN and a longitude of 3.9684oE
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