Abstract
Molecular characterization is used to characterize the organism at the molecular level without any effect of environment or development or physiological state of the organism. Molecular characterization is done using Primers they are fragments of DNA that can be identified with the whole genome. The main goal of this project is to characterize food borne pathogen such as Staphylococcus aureus which is an opportunistic pathogen and Escherichia coli present in milk which is a widely consumed food product These pathogens are associated in causing human diseases. These pathogens can also be found collecting and isolating the samples to find its morphology accordingly but it requires huge time and also in a morphological note there are many similar characteristics for the organisms so it is difficult to identify and characterize So, the characterization is done using primers in PCR (Polymerase Chain Reaction).This method takes very less time and also we can share the DNA fingerprinting data to the public health agencies regarding consuming of the particular product. Keywords: Primer, PCR, Human Diseases, Staphylococcus aureus, Escherichia coli
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