Abstract
Aim:Fascioliasis is one of the most zoonotic diseases with global extension. As the epidemiological distribution of Fasciola may lead to various genetic patterns of the parasite, the aim of this study is to identify Fasciola hepatica based on spermatogenesis, and phylogenetic analysis using mitochondrial (nicotiamide adenine dinucleotide dehydrogenase subunit I [ND1] and cytochrome oxidase subunit I) gene marker.Materials and Methods:In this study, 90 F. hepatica collected from 30 cattle at slaughterhouse located in three different geographical locations in the North-East of Iran were evaluated based on spermatogenetic ability and internal transcribed spacer 1 gene restriction fragment length polymorphism pattern. Genetic diversity and phylogenetic relationship using mtDNA gene marker for the isolates from the North-East of Iran, and other countries were then analyzed.Results:Partial sequences of mtDNA showed eight haplotypes in both genes. The phylogenic analysis using neighbor joining as well as maximum likelihood methods showed similar topologies of trees. Pairwise fixation index between different F. hepatica populations calculated from the nucleotide data set of ND1 gene are statistically significant and show the genetic difference.Conclusion:F. hepatica found in this region of Iran has different genetic structures through the other Fasciola populations in the world.
Highlights
Fasciolosis is a zoonotic parasitic disease that occurring in domestic grazing animals, affected on livestock economy by decrease in output products
F. hepatica found in this region of Iran has different genetic structures through the other Fasciola populations in the world
The causative species most commonly implicated of fasciolosis are Fasciola hepatica and Fasciola gigantica that F. hepatica is distributed worldwide, F. gigantica is fixed to warm parts and has been present in Africa along with South and Southeast Asia [2,3]
Summary
Fasciolosis is a zoonotic parasitic disease that occurring in domestic grazing animals, affected on livestock economy by decrease in output products. The causative species most commonly implicated of fasciolosis are Fasciola hepatica and Fasciola gigantica that F. hepatica is distributed worldwide, F. gigantica is fixed to warm parts and has been present in Africa along with South and Southeast Asia [2,3]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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