Abstract
BackgroundMembrane vesicles released by neoplastic cells into extracellular medium contain potential of carrying arrays of oncogenic molecules including proteins and microRNAs (miRNA). Extracellular (exosome-like) vesicles play a major role in cell-to-cell communication. Thus, the characterization of proteins and miRNAs of exosome-like vesicles is imperative in clarifying intercellular signaling as well as identifying disease markers.MethodsExosome-like vesicles were isolated using gradient centrifugation from MCF-7 and MDA-MB 231 cultures. Proteomic profiling of vesicles using liquid chromatography-mass spectrometry (LC-MS/MS) revealed different protein profiles of exosome-like vesicles derived from MCF-7 cells (MCF-Exo) than those from MDA-MB 231 cells (MDA-Exo).ResultsThe protein database search has identified 88 proteins in MDA-Exo and 59 proteins from MCF-Exo. Analysis showed that among all, 27 proteins were common between the two exosome-like vesicle types. Additionally, MDA-Exo contains a higher amount of matrix-metalloproteinases, which might be linked to the enhanced metastatic property of MDA-MB 231 cells. In addition, microarray analysis identified several oncogenic miRNA between the two types vesicles.ConclusionsIdentification of the oncogenic factors in exosome-like vesicles is important since such vesicles could convey signals to non-malignant cells and could have an implication in tumor progression and metastasis.
Highlights
Membrane vesicles released by neoplastic cells into extracellular medium contain potential of carrying arrays of oncogenic molecules including proteins and microRNAs
It has been reported that the extracellular vesicles derived from two breast cancer cell lines, MCF-7 and 8701-BC, carry several antigens including those expressed on the cell surface such as members of integrin family, tumor associated antigens, HLA class I molecules, matrix metalloproteinase-9, and tissue inhibitors of metalloproteinase-1 [16]
Quantitative analysis indicates that MCF-7 derived exosome-like vesicles (MCF-Exo) and MDA-MB231 derived exosome-like vesicles (MDA-Exo) exhibit relatively similar size with the diameter ranging from 80 to 200 nm
Summary
Membrane vesicles released by neoplastic cells into extracellular medium contain potential of carrying arrays of oncogenic molecules including proteins and microRNAs (miRNA). Exosome-like vesicles are present in body fluids such as synovial fluid, saliva, urine, semen, breast milk, and blood [4,5,6,7,8,9]. These vesicles have gained much attention for their important role in intercellular communication [3,10]. In the field of breast cancer research, the MCF-7 and MDA-MB 231 cell lines have been widely studied and characterized, there is no study analyzing miRNA and proteomics in their exosome-like vesicles
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