Molecular characterization of exon 3 of caprine myostatin gene in Marwari goat.

Jai Prakash Khichar,Kiran Kiran,Mohammad Ashraf,Ajay Singh Dewna,Prakash Prakash,Gyan Chand Gahlot,Vijay Kumar Agrawal

doi:10.14202/vetworld.2016.676-679

Jai Prakash Khichar, Kiran Kiran + Show 5 more

Open Access

https://doi.org/10.14202/vetworld.2016.676-679

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Abstract

Aim:To estimate genetic variability in exon 3 of caprine myostatin gene in Marwari goats.Materials and Methods:A total of 120 blood samples from unrelated Marwari goats were randomly collected from different villages of Bikaner (Rajasthan), India. Genomic DNA was extracted from whole blood using blood DNA isolation kit (Himedia Ltd.) as per manufacturer’s protocol. The quality of extracted genomic DNA was checked on 0.8% agarose gel. Specifically designed a primer set for caprine myostatin (MSTN) gene (Genebank accession no. DQ167575) was used to amplify the exon 3 region of MSTN gene in Marwari goat. The genetic variability in exon 3 of MSTN gene in Marwari goat was assessed on 8% polyacrylamide gel electrophoresis to detect single strand conformation polymorphism (SSCP) pattern.Results:The exon 3 of MSTN gene in Marwari goat showed two types of conformation patterns on 8% polyacrylamide gel. One of the patterns showed only two bands and was considered as genotype AA, whereas another pattern having an extra band was designated as genotype AB. The frequencies of AA and AB genotype for exon 3 region of MSTN gene were calculated as 0.90 and 0.10, respectively.Conclusion:Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through SSCP analysis. This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan.

Highlights

Marwari goat is a major meat breed of Rajasthan (India) that is well-adapted to the arid environment and can tolerate higher salt loads and requires less water
Low level of polymorphism was observed at exon 3 region of MSTN gene in Marwari goat through single strand conformation polymorphism (SSCP) analysis
This information could be utilized in future breeding plan to exploit the unique characteristics of Marwari goat of Rajasthan

Summary

Introduction

Marwari goat is a major meat breed of Rajasthan (India) that is well-adapted to the arid environment and can tolerate higher salt loads and requires less water. The unique characteristics of this breed require its molecular characterization for growth traits. Myostatin (MSTN) or growth and differentiation factor 8 is a member of the transforming growth factor-β superfamily that plays an important role in the regulation of muscle growth and meat quality [2]. The three exons and two introns of MSTN gene encode a glycoprotein that is expressed widely in skeletal muscles [3]. MSTN gene plays a critical role in myogenic differentiation and results in failure of myoblast to differentiate into myotubes through inhibition of MyoD activity and expression via Smad3 [4]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

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