Abstract
The characterization of microbial populations that act in the anode compartment of the MFC allows to obtain pure cultures, the same ones that allow obtaining better electrical energy voltages. Therefore, the objective of this research was to molecularly characterize axenic cultures from an anodic Bio-film formed by exo-electrogenic bacteria from Páramo soil for its application for treating leachate from fruits and vegetables from the Riobamba wholesale market in central Ecuador through cells of microbial fuel. The novelty of this research is the finding of potential microorganisms from high Andean soils that can reduce pollutant loads from wastewater with energy recovery. For the experimentation, the Delftia acidovorans bacterium obtained from molecular identification of nine species and a Páramo soil solution was selected as inoculums for two treatments in triplicate. Simple configuration cells of 510 mL volume were built and data acquisition system was used to automatically collect voltage values on a computer for 15 cycles. The statistical analysis determined that there are no significant differences in the production of voltage and removal of pollutants in both treatments, obtaining similar percentages of removal of organic matter measured in BOD5, COD and N-NH4. However, treatment with Páramo soil yielded higher removal percentages. It was concluded that the axenic and heterotrophic cultures used with continuous flow feeding favored the increase in pH, the production of bioelectricity, and removal of organic matter. Keywords: Páramo Soils, Molecular Identification, Exo-Electrogenic Bacteria, Microbial Fuel Cell, Leachate Treatment DOI： https://doi.org/10.35741/issn.0258-2724.58.3.33
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