Abstract
<p><strong>Objective: </strong>Molecular characterization of <em>Hedychium coronarium </em>from 4 different populations of Odisha using 9 inter simple sequence repeats and 15 random amplified polymorphic DNA markers to indicate the closeness of species and hybrids quickly and efficiently.</p><p><strong>Methods: </strong>A dendrogram was constructed through sequential agglomerative hierarchial and nested (SAHN) clustering and un-weighted pair group method with arithmetic mean (UPGMA) analysis using Jaccard’s similarity coefficient of combined markers using this particular species.<strong></strong></p><p><strong>Results: </strong>Two major clusters were found, i.e., cluster-I (Malkangiri-1, Phulabani-1, Phulabani-3, Malkangiri-2, Khurda-1, Khurda-2, Khurda-3, Angul-3, Angul-1, Angul-2 and Phulabani-2) and cluster-II (Malkangiri-3). The clustering pattern also revealed moreover the extent of genetic similarity between germplasms collected from four different regions population. <strong></strong></p><p><strong>Conclusion: </strong>The potential of this technique would be further realised to the fullest extent for the identification and tagging of the important novel gene in different taxa, unexplored yet, thus facilitating the improvement of desired taxa of Zingiberaceae. The findings would be of immense enough significance for complementing the strategies of conservation and characterization of these important taxa of Zingiberaceae following modern biotechnological approach.</p>
Highlights
There are several molecular markers which have been regularly used for studying genetic relations, population genetics and genetic characterization in different plant groups and crop cultivars
The molecular markers are not influenced by the external environmental factors unlike that of morphological markers accurately testify genetic relationship between and among plant groups
RAPD have been used for measuring genetic diversity in several plant species like apple [2], wheat [3], Piper [4], Triticum [5], Gossypium [6], Oryza [7], Barley [8], Sugarcane [9], Cymbopogon [10], Tea [11], Soybean [12], Banana [13], Broccoli [14]
Summary
There are several molecular markers which have been regularly used for studying genetic relations, population genetics and genetic characterization in different plant groups and crop cultivars. For proper conservation programme characterization of plants genetically is necessary These are routinely used as because it is more reliable, less time consuming and easy to handle in comparison with morphological and biochemical markers. Number of molecular markers (random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), inter-simple sequence, repeats (ISSR), simple sequence repeats (SSR), isozymes, proteins, etc.) are being widely used for germplasm evaluation, measuring genetic diversity, genetic mapping, for assessing genetic relationship among different taxa and for the detection of genetic changes caused due to mutation or genetic engineering. There was no report about the molecular characterization of Hedychium coronarium from eastern India This molecular technique could be used in the study of genetic diversity of endangered plant species to conserve the particular species
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