Molecular Characterization of Different Salmonella Enterica Serotypes Isolated From Frozen Meat in Minoufiya Governorate

Abstract

Salmonella is one of the most important causative agents of food poisoning and gastroenteritis in humans. This study spot highlights on isolation, identification and molecular characterization of salmonella serovars from imported frozen meat using the convential and modern molecular tools. Methods: A cross-sectional study was carried out on 100 samples of frozen meat collected from different supermarkets from Minufiya governorate, Egypt. Results: The prevalence of Salmonella were 6%. Serotyping of the obtained salmonella isolated revealed that Salmonella enteritidis, Salmonella typhimurium and Salmonella .paratyphi were the prevalent serotypes in the examined samples. S. typhimurium, only 3 samples (3%) ,Salmonella enteritidis was isolated from only 2 sample (2%) and S.paratyphi only 1 samples (1%). The application of conventional PCR for the six obtained isolates of Salmonella serotypes using universal gene (invA) was effective tool for identification and genotypic of pathogenic Salmonella serotypes. Conclusions: This study concluded that Salmonella is among the most important food borne pathogens worldwide contaminating a wide range of animal products including meat products. Also indicated that the cPCR was specific and rapid method for identification and genotyping of pathogenic salmonella serotypes.