Abstract
The present study was conducted during the period from September 2015 until February 2016. 100 fecal samples were collected from 60 sheep and 40 goats for diagnosis of Cryptosporidium parasite from diverse areas in Al-Qadisiyah province. The study amid to know the genetic characters of Cryptosporidium spp. parasite by using a molecular technique such as the nested polymerase chain reaction and DNA sequencing analyzed by phylogenetic tree to identify the parasite species. This study was done on the sheep and goat at first time in the middle region of Iraq and the identified species were recorded in NCBI-Genbank database. In sheep, the results of positive infected samples was (40%) while, in the goats were (32.5%), the DNA sequencing and phylogenetic analysis method based on small ribosomal RNA gene (18s rRNA) for Cryptosporidium species typing. The results were conducted by Neighbor-Joining phylogenetic tree analysis method and the 18s rRNA gene sequences were confirmed by using NCBI-BLAST data analysis in order to compare with NCBI submitted selected references isolates of (18s ribosomal RNA) gene in Cryptosporidium spp. parasites. Our finding in present study appeared to follow spp. (C. parvum, C. hominis, C. andersoni, C. ubiquitum, C. xiaio and C. suis). These identified species which primary affected sheep and goats as mentioned in previous studies when compared with newly Iraq isolates strains.
Highlights
Materials and MethodsThe apicomplexan protozoan parasiteThe nested PCR technique was performedCryptosporidium infects an extensive range of for detection Cryptosporidium spp. based 18S farm animals, primarily causing gastritis, ribosomal rRNA gene from sheep and goat diarrhea, and/or catarrhal respiratory signs (1 fecal samples by multiple steps: The feces and 2)
The results were conducted by Neighbor-Joining phylogenetic tree analysis method and the 18s rRNA gene sequences were confirmed by using NCBI-BLAST data analysis in order to compare with NCBI submitted selected references isolates of
European countries: C. parvum [6] and Cryptosporidium spp based on 18s rRNA gene recently C. xiaio from two kids suffering from by using Nested PCR technique were designed diarrhea minimum than 21 days old [7]
Summary
The present study was conducted during the period from September 2015 until February 2016. The study amid to know the genetic characters of Cryptosporidium spp. parasite by using a molecular technique such as the nested polymerase chain reaction and DNA sequencing analyzed by phylogenetic tree to identify the parasite species. The results of positive infected samples was (40%) while, in the goats were (32.5%), the DNA sequencing and phylogenetic analysis method based on small ribosomal RNA gene (18s rRNA) for Cryptosporidium species typing. The results were conducted by Neighbor-Joining phylogenetic tree analysis method and the 18s rRNA gene sequences were confirmed by using NCBI-BLAST data analysis in order to compare with NCBI submitted selected references isolates of (C. parvum, C. hominis, C. andersoni, C. ubiquitum, C. xiaio and C. suis) These identified species which primary affected sheep and goats as mentioned in previous studies when compared with newly Iraq isolates strains
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