Abstract
BackgroundThe aim of this study was to characterize class 1,2 and 3 integrons in clinical MDR Klebsiella pneumoniae isolates in Kashan, Iran.MethodsOne hundred-eighty one Klebsiella pneumoniae were recovered from clinical specimens during November 2013 to October 2014. Antimicrobial susceptibility patterns were determined by disk diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines for detection of MDR strains. Of the 181 Klebsiella pneumoniae, 146 (80.7%) of isolates were isolated from nosocomial infected patients and 150 (82.9%) identified as MDR isolates. The PCR amplification was used to show presence of class 1, 2 and 3 integrons among MDR strains. The PCR method and sequencing were used for evaluation of cassette content of integrons.ResultsOf the MDR K. pneumoniae isolates, 150 (100%) and 55 (36.7%) carried intI1 and intI2 genes, respectively. None of the MDR Klebsiella pneumoniae isolates carried class 3 integrons. Amplification of conserved segment (CS) of class 1 and class 2 integrons revealed 10 different arrays including: No. cassette; dfrA5, dfrA30; aadA2; aadA2, dfrA12; dfrA17, aadA5, aadA4; dfrA5, dfrA30, aadA2; dfrA5, dfrA30, aadA2, dfrA12, dfrA5, dfrA30, dfrA17, aadA5, aadA4; aadA2, aadA2, dfrA12; dfrA5, dfrA30, aadA2, aadA2, dfrA12 and 4 arrays including: No. cassette; aadA1; dfrA1-sat1; aadA1, dfrA1-sat1, respectively.ConclusionsThe finding of present study revealed a high prevalence of integrons especially class 1 among MDR K. pneumoniae isolates from nosocomial infections in Kashan, which led to rapid extension of MDR strains.
Highlights
The aim of this study was to characterize class 1,2 and 3 integrons in clinical Multi-drug resistant (MDR) Klebsiella pneumoniae isolates in Kashan, Iran
The structure of class 1 integrons is consisted of two conserved regions, including 3′ conserved segment (3′ CS) and 5′ conserved segment (5′ CS), as well as internal gene cassettes that encode antimicrobial resistance genes [7]
Several studies have documented the prevalence of integrons in MDR K. pneumoniae isolated from clinical specimens in Iran [9, 10], there is little information regarding the association between class 2 and 3 integrons and MDR, cassettes contents of integrons in K. pneumoniae isolates from nosocomial infected patients in our region
Summary
The aim of this study was to characterize class 1,2 and 3 integrons in clinical MDR Klebsiella pneumoniae isolates in Kashan, Iran. Class 1 integrons are the most prevalent and have been frequently reported in clinical isolates of gram negative bacteria including K. pneumoniae [7]. Several studies have documented the prevalence of integrons in MDR K. pneumoniae isolated from clinical specimens in Iran [9, 10], there is little information regarding the association between class 2 and 3 integrons and MDR, cassettes contents of integrons in K. pneumoniae isolates from nosocomial infected patients in our region. The present study proposed to characterize class 1, 2 and 3 integrons in clinical MDR K. pneumoniae isolates in Kashan, Iran
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