Molecular characterization of chicken anemia virus circulating in chicken flocks in egypt.

Abd Elnaby Y Metwally,Alaa G Abd El-Razak,Mohammed Aboelkhair

doi:10.1155/2014/797151

Abstract

Introduction. Although many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. In the present study, four nucleotide sequences of detected CAV were genetically characterized. Methods. These nucleotide sequences were obtained from commercial chicken flocks in two different locations of Egypt during 2010. The target region for sequencing was 675 bp nucleotide of partial coding region of VP1 protein. The nucleotide and deduced amino acid sequences of the detected CAV were aligned and compared to worldwide CAV isolates including commonly used vaccine strains. Phylogenetic analysis of these sequences was also carried out. Results. Our results showed that all the Egyptian CAV sequences were grouped in one group with viruses from diverse geographic regions. This group is characterized by amino acids profile 75I, 97L, 139Q, and 144Q in VP1. The phylogenetic and amino acid analyses of deduced amino acid indicated that the detected CAV sequences differ from CAV vaccine strains. Conclusion. This is the first report that describes molecular characterization of circulating CAV in Egypt. The study showed that the detected CAV, in Egypt are field viruses and unrelated to vaccine strains.

Highlights

Many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published
A number of CAV sequences have been reported in African continent, for example, Nigeria [5], Central African Republic, and Cameroon [17], and more recently from South Africa [18]
Vp1 gene sequence is commonly used to determine the relationship of different CAV isolates due to the fact that most of the amino acid substitutions between isolates lie in vp1 gene and in the N-terminal half of vp1 gene [6, 19]

Summary

Introduction

Many previous studies reported detection of chicken anemia virus (CAV) in Egypt since 1990, genomic characterization of this circulating CAV has not been published. Four nucleotide sequences of detected CAV were genetically characterized. The nucleotide and deduced amino acid sequences of the detected CAV were aligned and compared to worldwide CAV isolates including commonly used vaccine strains. Phylogenetic analysis of these sequences was carried out. Our results showed that all the Egyptian CAV sequences were grouped in one group with viruses from diverse geographic regions This group is characterized by amino acids profile 75I, 97L, 139Q, and 144Q in VP1. Mortalities and morbidities due to CAV infection may reach 55% and 80%, respectively [8]

Objectives

Methods

Results

Discussion

Conclusion