Molecular Characterization of blaTEM and blaCTX-M in ESBLs producing Escherichia coli and Salmonella spp. in Poultry farm and Klebsiella pneumoniae and Proteus spp. in Klang River in Malaysia

Abstract

Event Abstract Back to Event Molecular Characterization of blaTEM and blaCTX-M in ESBLs producing Escherichia coli and Salmonella spp. in Poultry farm and Klebsiella pneumoniae and Proteus spp. in Klang River in Malaysia Nagaraja Suryadevara1*, Kai Boon Yong1, Sharanya Rajan1, Yoke Ing Kwan1 and Michelle Fong Ting Lim1 1 Mahsa University, Malaysia Introduction: Multidrug resistance bacteria (MDR) has emerged as a public health threat nowadays. High level of resistance to beta-lactam antibiotics among Enterobacteriaceae was conferred by Extended Spectrum Beta-Lactamase (ESBL) in the poultry farm. The waste material of the livestock could pollute the river, leading to the aquatic environment as a reservoir of antimicrobial resistance. Methods: The physiochemical properties of water samples were determined. Gram-negative microorganisms were identified using differential media and biochemical testing. Confirmed bacterial isolates were tested for multidrug resistance by using Kirby Bauer method. The ESBL strains were determined phenotypically using Combination Disc Test (CDT) and Double Disc Synergy Test (DDST). Bacterial genomic DNA was extracted from positive ESBL strains, blaTEM and blaCTX-M genes were amplified using Polymerase Chain Reaction (PCR). The amplified products were sequenced to identify the mutated genes in MDR strains. Results: Out of 88 samples collected from poultry farm, 73 (83.0%) E.coli and 59 (67.0%) Salmonella spp. were identified. Of 50 water samples from Klang River, 31 (62.0%) Klebsiella pneumoniae and 13 (26.0%) Proteus spp. were found. In total, 11 (15.1%) E.coli, 14 (23.7%) Salmonella spp., 12 (38.7%) Klebsiella pneumoniae, and 7 (53.8%) Proteus spp. were MDR strains. From there, 8 (72.7%) E.coli, 7 (50.0%) Salmonella spp., 5 (16.1%) Klebsiella pneumoniae and 5 (38.5%) Proteus spp. were ESBL positive strains. The genes blaTEM and blaCTX-M were seen exhibited in 3 (42.9%) and 7 (87.5%) E.coli , 1(14.3%) and 5(71.4%) Salmonella spp., 1 (20.0%) and 4(80.0%) Klebsiella pneumoniae and 1 (20.0%) and 4 (80.0%) Proteus spp., respectively. Conclusion: Classic blaTEM and blaCTX-M genes were present in E.coli and Salmonella spp. from poultry samples; and Klebsiella pneumoniae and Proteus spp. from river water samples. However, blaCTX-M gene was seen more prominent compared to blaTEM gene in the isolated ESBL strains. Acknowledgements Author would like to thank the MAHSA University Management for providing us the necessary laboratory facilities and equipment through out the research. Keywords: MDR - (multi drug resistant), ESBL - Extended-spectrum beta-lactamase, Bla TEM, bla CTX -M1, DDST, CDT Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Miscellaneous Citation: Suryadevara N, Yong K, Rajan S, Kwan Y and Ting Lim M (2019). Molecular Characterization of blaTEM and blaCTX-M in ESBLs producing Escherichia coli and Salmonella spp. in Poultry farm and Klebsiella pneumoniae and Proteus spp. in Klang River in Malaysia. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00091 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 22 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Nagaraja Suryadevara, Mahsa University, Kuala Lumpur, Malaysia, nagaraja@mahsa.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nagaraja Suryadevara Kai Boon Yong Sharanya Rajan Yoke Ing Kwan Michelle Fong Ting Lim Google Nagaraja Suryadevara Kai Boon Yong Sharanya Rajan Yoke Ing Kwan Michelle Fong Ting Lim Google Scholar Nagaraja Suryadevara Kai Boon Yong Sharanya Rajan Yoke Ing Kwan Michelle Fong Ting Lim PubMed Nagaraja Suryadevara Kai Boon Yong Sharanya Rajan Yoke Ing Kwan Michelle Fong Ting Lim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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