Abstract
Barley possesses the highest level of salt tolerance among cereals, thus it is used as an important species to investigate mechanisms involved in salt tolerance. The present investigation was conducted to study the effect of salt stress by different NaCl concentrations (0, 9000, 12000, and 15000 ppm) on proline production and it was found that there is a positive correlation between proline production and salinity. Also study the effect of salinity on BADH-1 gene expression by Real-Time PCR,where this gene was performed and it was aligned in GenBank. The sequencing was translated into amino acid sequences and these amino acid sequences were aligned in GenBank. Finally, the secondary structure of BADH-1 enzyme was determined from the most tolerant barley (Giza 2000 and Wadi Sedr) and sensitive barley Giza 129 cultivars, then sequencing translated into amino acid sequences and these sequences were aligned in GenBank. Finally, the secondary structure of BADH-1 enzyme was determined. These isolated genes were submitted in GenBank under accession numbers KX433169, KX342849, KX342850, KX342851, KX433170, and KX433171.
Highlights
Salinity is one of the serious problems affecting one-third of the irrigation land nearly950 million ha land in the world, it has an important effect on plant growth and productivity of modern cultivars (Maas and Hoffman, 1977; Babu et al, 2007 and Munns, 2002)
Study the effect of salt stress by different NaCl concentrations on proline production from each cultivar and study the effect of salinity on Betaine aldehyde dehydrogenase 1 ( Betaine Aldehyde Dehydrogenase1 (BADH-1)) gene expression by using Real-Time polymerase chain reaction (PCR), where this gene was performed and it was aligned in GenBank
Molecular characterization of (BADH-1) gene was carried out from the most tolerant and sensitive cultivar, where, the sequencing of the gene was translated into amino acid sequences, which were aligned in GenBank, the secondary structure of BADH-1 enzyme was detected
Summary
Salinity is one of the serious problems affecting one-third of the irrigation land nearly950 million ha land in the world, it has an important effect on plant growth and productivity of modern cultivars (Maas and Hoffman, 1977; Babu et al, 2007 and Munns, 2002). Salinity is one of the serious problems affecting one-third of the irrigation land nearly. In Egypt, there are about two million feddans of the irrigated land adversely affected by the accumulation of salts. This environmental stress is a complex trait controlled by a large number of genes which makes them elusive to selection for tolerance by conventional breeding programs (Abo-Elenin et al, 1981). For the overcoming of salt stress problems, plants have evolved complex mechanisms that contribute to the adaptation to osmotic and ionic stress caused by high salinity. During the onset and development of salt stress within a plant, all the major processes such as compatible osmolytes, protein synthesis, and lipid metabolism are affected. The resistance or sensitivity to salt stress depends on the species, the genotype, and the development age of the plants
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