Molecular characterization of andalas tree dioecious plant [Morus macroura Miq.] using SRAP marker

Abstract

Sumatran Mulberry [Morus macroura var. Macroura] is the mascot [identity] flora of West Sumatra. Clarification of individual sex and genetic variation is necessary for its conservation. The SRAP [Sequence Related Amplified Polymorphism] marker system is one of the powerful molecular tools for clarification of individual sex and estimating its genetic diversity of plant species. The purpose of this study was to examine the male and female individuals in Andalas [Morus macroura] trees and to analyze their genetic variation. The fifteen individuals [accessions] of Morus macroura from three locations in West Sumatra [Limau Manis, Padang, Lubuak Gadang, South Solok and Andaleh, Tanah Datar] were collected to provide the DNA samples. A combination of seven primers was used to amplify the fifteens of DNA samples. Specific bands for the sex were examined in selected individuals, and Geneticvariation with four parameters: Number of allele [na], Number of effective allele [ne], Gene Diversity [h]; Shannon’s Information Index [I] were analyzed. The PCR Test indicated that Fifty fragments were obtained from the amplification of DNA samples using seven combinations of primers. The most number of fragments were found in the combination of primer B and E. The specific band at The 20 bp and 30 fragments were useful to distinguish the sex of the examined individual trees. The genetic variation of Morus macroura was moderate low [na=1.540; ne=1.273; h=0.168; and I=0.259]. Genetic diversity of Limau Manis Population [h=0.241] was higher than Lubuak Gadang [h=0.116] and Andaleh population [h=0.148].