Abstract
Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both blaIMP–26 and tet(A) variant in clinical Klebsiella pneumoniae KP-1572. MIC results showed that K. pneumonia KP-1572 was resistant to a wide range of antimicrobials. The blaIMP–26 and tet(A) variant were located on an identical plasmid, which was indicated by S1-PFGE and southern blotting hybridization and can be successfully transferred by electroporation. Whole-plasmid sequencing and analysis revealed that a 142,993-bp-sized plasmid, designated pIMP1572, contains an IncFIIk backbone and a variable region harboring blaIMP–26 and tet(A) variant. The plasmid pIMP1572 was apparently originated from a tet(A)-carrying IncFIIk plasmid but with a deletion length of 6,216-bp and a multiple drug resistance region (MDRR) insertion of 25,259 bp. The plasmid pIMP1572 in the present study represents the first report of the IncFIIk plasmid co-carrying blaIMP and tet(A) variant, which should be monitored.
Highlights
Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an increasing problem worldwide (Nordmann et al, 2011; Munoz-Price et al, 2013)
Klebsiella pneumoniae KP-1572 exhibited a multiple drug resistance (MDR) profile for a wide range of antimicrobial agents, including imipenem, meropenem, aztreonam, ceftazidime, gentamicin, tetracycline, tigecycline, and colistin, while it was susceptible to amikacin and fosfomycin (Table 1)
Resistance gene screening and sequencing revealed that K. pneumonia KP-1572 co-carried the carbapenem-resistance gene blaIMP−26 variant and tigecycline-resistance gene tet(A) variant
Summary
Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an increasing problem worldwide (Nordmann et al, 2011; Munoz-Price et al, 2013). The previously described tet(A) variant (Yao et al, 2018) and recently identified tet(X) variants, such as tet(X3), tet(X4), tet(X5), and tet(X6) (He et al, 2019; Sun et al, 2019; Wang L. et al, 2019a; Liu et al, 2020), have been reported to mediate the low-level and high-level tigecycline resistance, respectively. Both tet(A) variant and tet(X) variants are mobilized, indicating that they are posing a higher threat to public health. We characterized an IncFIIk plasmid co-carrying blaIMP−26 and tigecycline-resistant gene tet(A) variant in a clinical K. pneumoniae isolate which displayed resistance to carbapenems and tigecycline
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