Molecular characterization of an alternative model of inflammation (P1347)

Abstract

Abstract Non-steroidal anti-inflammatory drugs (NSAIDs) for food-producing animals such as swine need to control systemic and local inflammatory responses. However, there are no NSAIDs approved to control inflammation in swine due to an absence of qualified measures of efficacy needed to substantiate those claims. The absence of systems and approaches to substantiate a claim to control inflammation has led to the total absence of approved products for swine. The goal of this study was to develop an in vitro carrageenan model of local inflammation for use in studies of novel non-steroidal anti-inflammatory drug efficacy, and to determine if there are common pathways/biomarkers for local and systemic inflammatory responses. We evaluated carrageenan induced stimulation and its capacity to affect previously identified systemic inflammatory biomarkers in swine, using swine whole blood cultures. We found that lambda carrageenan increases the production of IL-8 and MCP-1 via ELISA. Conversely, TNF-α and SAA demonstrated a no change in production. Additionally, Phosphokinase-3, Monocyte Chemotactic Protein-1, Alveolar Macrophage Chemotactic -II showed increased gene expression. These results suggest lambda carrageenan impacts ubiquitination on immune cells. These results also demonstrate that carrageenan initiates an inflammatory response that differs from that induced by endotoxin in terms of the pathways and gene products altered as a consequence of carrageenan exposure.