Abstract
Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
Highlights
The genus Digitaria includes approximately 300 species, distributed in tropical and subtropical regions of both hemispheres (CANTO-DOROW, 2001)
In the State of São Paulo, this genus is represented by 14 characterized species (CANTO-DOROW, 2001; DIAS et al, 2003) and the visual differentiation of these species in the field is difficult due to the morphological similarities among them (DIAS et al, 2003), which are basically differentiated by spike characteristics (DIAS et al, 2005a)
Research on genetic variability with the use of molecular markers, such as RAPD and PCR-RFLP, permit to estimate the genetic variability, among and within species, and serve as the starting point for studies to manage these plants, since, when combined with sequencing, they allow the localization of resistance genes as well as possible mutations related to the onset of resistant individuals in some species
Summary
The genus Digitaria includes approximately 300 species, distributed in tropical and subtropical regions of both hemispheres (CANTO-DOROW, 2001). Crabgrass (Digitaria nuda, D. ciliaris and D. horizontalis) are weeds that normally infect the sugar cane crop in Brazil, where they cause quantitative and qualitative reductions in the production. It is important that sugar cane farms make the correct identification of the species of crabgrass in its areas and use objective strategies of management for each one of the species (DIAS et al, 2007). The management of weed in the sugar cane crop is carried out by combined chemical (application of herbicides) and mechanical (cultivation) methods. The usage of herbicides is an indispensable and common practice in sugar cane plantations, as a result of the extensiveness of the planted areas and the high cost of manual labor
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