Abstract
Nested polymerase chain reaction was conducted to identify the phytoplasma associated with grassy shoot disease (GSD) of sugarcane in the Gorakhpur district of India. A 1.25 kbp DNA fragment encoding the phytoplasma 16S rKNA gene was consistently amplified by nested PCR. The results showed that samples of diseased plants from two sugarcane varieties gave the same restriction fragment length polymorphism (RFLP) patterns with two restriction enzymes (Hae III and Rsa I) similar to that of other known sugarcane phytoplasmas. This is the first report on molecular characterization of phytoplasma associated with GSD of sugarcane in India.
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