Abstract
In plants, catalases are encoded by a multigene family and are predominantly localized in the peroxisomes and glyoxysomes for scavenging H<sub>2</sub>O<sub>2</sub>. A full-length cDNA encoding <em>Catalase</em> (<em>VsCat</em>) was isolated from <em>Vicia sativa</em> using RT-PCR. The cDNA consisted of 1485 bp open reading frame (ORF) encoding a 494 amino acid polypeptide with a predicted molecular mass of 57.03 kDa and an estimated pI of 6.56. The predicted protein was highly homologues to other catalases from legume plants and contained typical catalytic active site, calmodulin binding domain, and internal peroxisomal targeting signal. Phylogenetic analysis revealed that VsCat is evolutionary close to faba bean and pea Cat1. The expression patterns of <em>VsCat</em> under different abiotic stresses and exogenous phytohormones were determined by quantitative RT-PCR<strong>. </strong>Compared to control plants, <em>VsCat</em> was differentially up-regulated in response to abiotic stresses and phytohormones. The expression analysis suggested that <em>VsCat</em> is involved in different abiotic stress responses. In future experiment, transgenic plants overexpressing <em>VsCat</em> might be a good choice to increase tolerance of forage plants to environmental stresses.
Highlights
IntroductionPlants subjected to biotic and abiotic stress factors exhibit different protective biochemical mechanisms, including decreased stomatal conductance and increased photoreduction of molecular oxygen, photorespiration, and excitation of electron transport chain at photosystem II (Asada, 1999; Ort & Baker, 2002)
Crop plants are greatly affected by environmental stresses
Plants subjected to biotic and abiotic stress factors exhibit different protective biochemical mechanisms, including decreased stomatal conductance and increased photoreduction of molecular oxygen, photorespiration, and excitation of electron transport chain at photosystem II (Asada, 1999; Ort & Baker, 2002). These protective mechanisms lead to increased formation of highly reactive forms of oxygen known as reactive oxygen species (ROS), such as superoxide anion (O2.–), hydroxyl radical (·OH), peroxyl radical (HOO–), hydrogen peroxide (H2O2) and singlet (1O2; Asada, 1999; Mittler, 2002)
Summary
Plants subjected to biotic and abiotic stress factors exhibit different protective biochemical mechanisms, including decreased stomatal conductance and increased photoreduction of molecular oxygen, photorespiration, and excitation of electron transport chain at photosystem II (Asada, 1999; Ort & Baker, 2002). These protective mechanisms lead to increased formation of highly reactive forms of oxygen known as reactive oxygen species (ROS), such as superoxide anion (O2.–), hydroxyl radical (·OH), peroxyl radical (HOO–), hydrogen peroxide (H2O2) and singlet (1O2; Asada, 1999; Mittler, 2002). These effects are collectively referred to as oxidative stress (Halliwell, 2006; Møller, Jensen, & Hansson, 2007)
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