Molecular Characterization and Validation of Micro-Satellite Markers Linked to Powdery Mildew Disease Resistance in Mini Core Germplasm of Urdbean [Vigna mungo (L.) Hepper

Abstract

Twenty-nine Urdbean [Vigna mungo (L.) Hepper] genotypes, including four check varieties 'Prasad,' 'Ujala,' 'PU-31' and 'OBG-31' were evaluated in the experimental farm of Odisha University of Agriculture and Technology, Bhubaneswar, India, for two seasons to assess these genotypes against powdery mildew disease incidence and to estimate the extent of genetic divergence and character association employing molecular markers. For molecular diversity studies five molecular markers i.e., MB-SSR238, VrCsSTS1, CEDG191, VrCsSSR1, CEDG166 were evaluated on the Urdbean genotypes. The results showed the association of VrCsSTS1 marker with the powdery mildew resistance gene in different genetic backgrounds. The genotypes 'OBG-31', 'Ujala,' 'Prasad,' and 'Nayagarh-I local' were resistant to powdery mildew disease, which can be further used for breeding programs.