Abstract
One grapevine ( Vitis vinifera L.) Adh gene, related to grapevine ripening and referred to here as GV- Adh 1, has been completely sequenced. We report in this paper the cloning strategy and the structural characteristics of this gene. Comparative analyses of the proximal region of GV- Adh 1 promoter with other promoters from Adh genes are presented. GV- Adh1 contains a 1140 nucleotide open reading frame encoding for a 380 amino acid polypeptide with a predicted molecular mass of about 41 kDa and a predicted isoelectric point of 6.9. Northern blot analysis detected Adh transcripts principally in ripening fruits, whereas constitutive or no expression was found in young expanding leaves, tendrils and roots. A large increase in Adh expression corresponds to the inception of berry ripening. Blot hybridization experiments show that in grapevine, Adh is coded by a small multigene family. Phylogenetic analysis is presented.
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