Abstract
In Anopheles stephensi AsSGU is a GPI-anchored protein, expressed in midgut periotrophic matrix. The GPI anchored proteins are involved in parasite invasion, protein-protein and protein-ligand interaction. Its function in Plasmodium ookinetes invasions in midgut provides it as new target candidate antigen for transmission blocking vaccines. Here the gene of 503 bp encoded for Assgu was amplified and submitted to GenBank (id: MN402757), encoded for 167 amino acid. The sequence The AsSGU composed of two domains, signal peptide and MBF2 domain, it was conserved in most of the Anopheles mosquito with similarity 65–83%. The 3-D structure of the protein was predicted based on thread modelling and validated with bioinformatic tools. The effective interaction of AsSGU with TLR4 conformed through molecular docking. Although these studies cleared the various aspects about the AsSGU, still there is need to validate experimentally in laboratory for its immunogenic behaviour and functional analysis.
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