Abstract
BackgroundCystic echinococcosis (CE) is a serious parasitic zoonosis caused by the larvae of the tapeworm Echinococcus granulosus. The development of an effective vaccine is one of the most promising strategies for controlling CE.MethodsThe E. granulosus 3-hydroxyacyl-CoA dehydrogenase (EgHCDH) gene was cloned and expressed in Escherichia coli. The distribution of EgHCDH in protoscoleces (PSCs) and adult worms was analyzed using immunofluorescence. The transcript levels of EgHCDH in PSCs and adult worms were analyzed using quantitative real-time reverse transcription PCR (RT-qPCR). The immune protective effects of the rEgHCDH were evaluated.ResultsThe 924-bp open reading frame sequence of EgHCDH, which encodes a protein of approximately 34 kDa, was obtained. RT-qPCR analysis revealed that EgHCDH was expressed in both the PSCs and adult worms of E. granulosus. Immunofluorescence analysis showed that EgHCDH was mainly localized in the tegument of PSCs and adult worms. Western blot analysis showed that the recombinant protein was recognized by E. granulosus-infected dog sera. Animal challenge experiments demonstrated that dogs immunized with recombinant (r)EgHCDH had significantly higher serum IgG, interferon gamma and interleukin-4 concentrations than the phosphate-buffered saline (PBS) control group. The rEgHCDH vaccine was able to significantly reduce the number of E. granulosus and inhibit the segmental development of E. granulosus compared to the PBS control group.ConclusionsThe results suggest that rEgHCDH can induce partial immune protection against infection with E. granulosus and could be an effective candidate for the development of new vaccines.Graphical abstract
Highlights
Cystic echinococcosis (CE) is a serious parasitic zoonosis caused by the larvae of the tapeworm Echinococcus granulosus
We evaluated the immunogenicity of recombinant (r)E. granulosus 3-hydroxyacyl-CoA dehydrogenase (EgHCDH) in a dog challenge model
Molecular characteristics The full-length cDNA sequence of EgHCDH consisted of 924 bp and encoded a protein of 308 amino acids with a predicted molecular mass of 34 kDa
Summary
Cystic echinococcosis (CE) is a serious parasitic zoonosis caused by the larvae of the tapeworm Echinococcus granulosus. Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by larvae of the tapeworm Echinococcus granulosus [1]. Xian et al Parasites Vectors (2021) 14:489 prevention and the use of anticoccidial drugs and vaccines. Drugs, such as praziquantel, are used mainly to deworm the definitive hosts (dogs) in order to block the transmission of E. granulosus. Deworming with these drugs is time-consuming and difficult to implement on a large scale in epidemic areas, and there is the risk of the development of drug resistance. The development of a dog vaccine against E. granulosus is urgently needed
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