Abstract
A total of thirty microsatellite molecular markers were used across 21 rice genotypes for their characterization and discrimination. The number of alleles per locus ranged from three (RM165, RM219, RM248, RM463, RM470 and RM517) to nine (RM223), with an average of 4.53 alleles across the 30 loci obtained in the study. The polymorphism information content (PIC) values ranged from 0.30 (RM219) to 0.84 (RM223) in all 30 loci. RM223 was found the best marker for the identification of 21 genotypes as revealed by PIC values. The frequency of the most common allele at each locus ranged from 24% (RM223 and RM334) to 81% (RM219). A two dimensional principal coordinate analysis (PCoA) with 21 genotypes showed that the genotypes Supper Basmoti, Basmati370, BasmatiD, Keora, Chinisakkora, Thakurbhog, Benaful, Kolgochi, Buchi, Awnedtapl and Kalijira-11 were found far away from centroid of the cluster than rest of the genotypes which placed around the centroid. The pair-wise genetic dissimilarity coefficients indicated that the highest genetic distance was obtained between Thakurbhog and Supper Basmoti (0.81) as well as between Benaful and Keora (0.81). Basmati (Basmoti D, Super Basmati, Basmati 370) and Kalijira (Kalijira 11, 12, 13, 14) genotypes had close similarity among them but showed wide dissimilarity with other local genotypes. Being grouped into distant clusters, SupperBasmoti, Thakurbhog, Keora, and Benaful could be utilized as potential parents for the improvement of fine grain aromatic rice varieties. Genotypes Kolgochi and Buchi (having zero dissimilairty) might be possessed same genetic background. The microsatellite marker based molecular fingerprinting could serve as a sound basis in the identification of genetically distant accessions as well as in the duplicate sorting of the morphologically close accessions.DOI: http://dx.doi.org/10.3329/bjpbg.v20i2.17028
Highlights
Consumers all over the world prefer aromatic rice due to its flavor and palatability
Genetic variability is the mainstay for the success of any breeding program assessing the level of genetic variation among the rice varieties or genotypes has been of great interest to the breeders
Rice taxonomists are interested in the rapid classification of different taxonomic groups, while breeders are concerned about the determination of usable agronomic variation in breeding programs (Zhou, 1995)
Summary
Consumers all over the world prefer aromatic rice due to its flavor and palatability. In Bangladesh, there are more than four thousand local landraces of rice, 1Seed Certification Agency, Ministry of Agriculture, Joydebpur, Gazipur 1701, 2 Biotech Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur 1701, Bangladesh adapted in different parts of the country, some of which have very nice quality, fineness, aroma, taste and protein contents (Kaul et al, 1982) These are generally low yielding, cannot compete with modern varieties of rice. Our study is involved with the aromatic and fine grain traditional landraces of rice locally cultivated by farmers those were either not used, or poorly used as parents in the Bangladeshi rice-breeding program. These germplasm can function as the source of alternative genetic pools to the improved varieties. 30 microsatellite markers were used against 21 fine grain aromatic genotypes to assess the extent of molecular diversity in the traditional aromatic rice genotype, to establish their genetic relationships and to evaluate their potential utility in the breeding programme
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