Abstract
Antimicrobial peptides (AMPs) are known to play a role as a first line of defence against microbial invasion. Liver Expressed Antimicrobial Peptides-2 (LEAP-2) is one of the AMPs. LEAP-2 includes four highly conserved cysteine residues and belongs to a cysteine-rich peptides group. We identified and characterized the molecular properties of LEAP-2 in rock bream. The expression levels of rock bream LEAP-2 (RbLEAP-2) in the 12 different tissues of healthy fish and the RbLEAP-2 expression pattern after infections with Edwardsiella piscicida (E. piscicida), Streptococcus iniae (S. iniae) and red seabream iridovirus (RSIV) were examined. This data provide that RbLEAP-2 plays an important role in innate immunity when rock bream is infected with a pathogen.
Highlights
Antimicrobial peptides (AMPs) are known to play a role as a first line of defence against microbial invasion
We identified and characterized the molecular properties of Liver Expressed Antimicrobial Peptides-2 (LEAP-2) in rock bream
The expression levels of rock bream LEAP-2 (RbLEAP-2) in the 12 different tissues of healthy fish and the RbLEAP-2 expression pattern after infections with Edwardsiella piscicida (E. piscicida), Streptococcus iniae (S. iniae) and red seabream iridovirus (RSIV) were examined. This data provide that RbLEAP-2 plays an important role in innate immunity when rock bream is infected with a pathogen
Summary
To obtain the full-length of RbLEAP-2 cDNA sequence, Generation Sequencing (NGS) analysis was performed on rock bream liver stimulated with Streptococcus iniae and Red Seabream Iridovirus (RSIV). The isolated base sequence of the full-length RbLEAP-2 cDNA and the corresponding amino acid sequences were determined using the BLASTX programme The molecular weight and isoelectric points (pI) were determined using the ProtParam tool on the ExPASy Proteomics Server (http://web.expasy.org/ protparam/). To identify the characteristic domains, Simple Modular Architecture Research Tool (SMART) (http://smart.embl-heidelberg.de/) was used. Multiple sequence alignments were analysed using ClustalW (http://www.genome.jp/tools/clustalw/). The tertiary structure prediction was performed using the I-Tasser server Rock breams were obtained from the Gyeongsangnam-do Fisheries Resources Research Institute, Korea. The weights and body lengths of these fish were 68.5 ± 10 g and 14.3 ± 1 cm, respectively. The fish were fed with commercial feed once a day
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