Abstract
Leucine zipper transcription factor like 1 (LZTFL1) is a member of the Bardet–Biedl syndrome gene family. LZTFL1-null mice show the phenotype of obesity, retinal degeneration, and abnormal cilia development. Functionally, LZTFL1 serves as a tumor suppressor and a negative regulator in the hedgehog signaling pathways. The biological function of mammalian LZTFL1 is partially addressed, but data on other model organisms are limited. Zebrafish (Danio rerio) is widely considered as a powerful model to understand the functions of genes implicated in obesity, disease, and cancer. In this study, LZTFL1 homologs were identified in zebrafish (zebrafish LZTFL1). The full-length cDNA of zebrafish LZTFL1 contained 897 bps encoding 298 amino acids. Zebrafish LZTFL1 displayed conserved domains of coil–coil and leucine zipper domain. PCR results showed that zebrafish LZTFL1 was widely distributed in various tissues. Western blot analysis further revealed that zebrafish LZTFL1 was detected to be ectopically expressed in HeLa cells with correct molecular weight. Fluorescence images showed as well that zebrafish LZTFL1 was localized in the cytoplasm. Furthermore, luciferase reporter assay indicated zebrafish LZTFL1 served as a negative regulator in the hedgehog signaling pathway. These data supported that zebrafish was a good model for understanding the biological roles of LZTFL1.
Highlights
Leucine zipper transcription factor like 1 (LZTFL1), called BBS17, is a member of the Bardet– Biedl syndrome (BBS) gene family (Sakurai et al, 2011; Khan et al, 2016)
The full-length cDNA of zebrafish LZTFL1 (GenBank accession number MK253678) is 897-bp-long, which encodes a putative protein of 298 amino acids (Figure 1A) with theoretical molecular weight of 34.468 kDa and isoelectric point of 5.71
The conserved feature was found in the signature of four leucine residues in the C-terminal of zebrafish LZTFL1 (Figure 2), which constituted the “leucine zipper pattern” among vertebrates (Figure 1C)
Summary
Leucine zipper transcription factor like 1 (LZTFL1), called BBS17, is a member of the Bardet– Biedl syndrome (BBS) gene family (Sakurai et al, 2011; Khan et al, 2016). Immunohistochemistry results indicated that LZTFL1 was highly expressed in the epithelial cells of normal tissues and showed significantly lower expression in the corresponding tumor samples including breast, liver, ovary, stomach, lung, and thyroid cancer (Wei et al, 2010; Wang et al, 2014). LZTFL1 expression level displayed significant correlation with longer survival time of cancer patients and had significantly inverse correlation with tumor metastasis (Wei et al, 2010, 2015; Wang et al, 2014). LZTFL1 may promote the maintenance of the differentiated state of lung epithelial cells via the suppression of the hedgehog signaling (HH) pathways (Wei et al, 2015)
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