Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A

H L Wang,S L Yang,K Li,Z M Zhu,H Wang,S T Feng

doi:10.5713/ajas.2006.953

Abstract

The eukaryotic elongation factor 1 A (EEF1A) participates in protein synthesis by forming the eEF1A GTP tRNA complex to deliver aminoacyl-tRNA to the A site of ribosomes. This study described cDNA sequences and partial genomic structure of porcine EEF1A1. The porcine EEF1A1 gene encoded a protein with 462 amino acids, which shared complete homology with human, chimpanzee and dog. The temporal expression pattern showed the diversity of EEF1A1 level in mRNA was relatively minor in prenatal embryo skeletal muscle, however, the expression decreased during aging after birth in skeletal muscle of the Chinese Tongcheng pig. The spatial expression patterns indicated that the gene expressed in skeletal muscle, heart, lung, liver, kidney, fat and spleen. In addition, we assigned the gene to porcine chromosome 1 using a radiation hybrid panel.

Highlights

A variety of molecules have been identified in the complicated network system of protein synthesis, such as Guanine triphosphate (GTP), ribosome, initial factors, elongation factors and termination factors
In protein translational elongation process, elongation factor 1 A (EF1A) contributes to the binding of aminoacyl-tRNA to the A site of ribosome at the presence of GTP and maintaining EF1A-GTP concentration in tRNA channeling which EF1βγ subunits were for exchange of GDP/GTP (Sanders et al, 1993)
Eukaryotic EF1A has structurally been identified as a GTP-binding protein characterized consensus sequences including G-X4-G-K, D-X2-G and N-K-X-D sequences (Dever et al, 1987), which the three highly conserved motifs showed the important roles in the regulatory process of translational elongation by some previous mutation

Summary

INTRODUCTION

A variety of molecules have been identified in the complicated network system of protein synthesis, such as Guanine triphosphate (GTP), ribosome, initial factors, elongation factors and termination factors. We isolated the full-length cDNA of porcine EEF1A1 gene from a skeletal muscle cDNA library of porcine embryos, where the porcine eukaryotic translation termination factor 1 (ETF1) gene was analyzed (Shao et al, 2005), characterized the genomic structure and patterns for temporal and spatial expression, identified the localization on porcine chromosome. All these will be the bases for a better understanding of EEF1A1 gene

MATERIAL AND METHODS

RESULTS AND DISCUSSION

CONCLUSION