Molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus).

Abstract

Ghrelin is a novel growth hormone (GH)-releasing peptide, which has been identified as an endogenous ligand for the GH-sretagogue receptor. The sequence and expression of ghrelin has been determined in many species. In this study, to reveal the molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus), the full-length DNA and cDNA encoding ghrelin were cloned from reindeer stomach using genome walking and rapid amplification of complementary deoxyribonucleic acid ends (RACE). The expression of ghrelin in almost all tissues was examined by real-time quantitative PCR (RT-qPCR). The 4076 bp amplicon of the ghrelin gene consisting of 4 exons and 3 introns was cloned from reindeer. Results of cDNA cloning and sequence analysis revealed that the full-length ghrelin cDNA was composed of 539 bp that included a 5'-untranslated region (46 bp), an open reading frame (ORF) (351 bp), and a 3'-untranslated region (142 bp). In addition, ghrelin was expressed in the all tissues examined, with the expression in the abomasum significantly higher than that in other tissues (p<0.05), followed by the pancreas, duodenum, testis and oesophagus. The results show that the expression of ghrelin in the reindeer gastrointestinal tract is extensive, suggesting its may have a role in regulating the digestive function.