Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus).

X J Wang,X D Guo,S Y Li,Y Liang,D J Liu,J J Shi,Z G Wang,M L Wu,J F Yang,Y F Wang

doi:10.5713/ajas.2011.11290

X J Wang, X D Guo + Show 8 more

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https://doi.org/10.5713/ajas.2011.11290

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Abstract

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal’s fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.

Highlights

Insulin-like growth factor-binding proteins (IGFBPs) are a family of secreted proteins that bind insulin-like growth factors I and II (IGFs I and II)
The IGFBP-5 gene cDNA (GenBank accession number JF720883) from the Inner Mongolia Cashmere goat was amplified by reverse transcription polymerase chain reaction (RT-PCR)
All features indicated are very similar to those found in previous research on IGFBP-5 in other species. These findings indicate that the IGFBP-5 gene has been correctly cloned from the Inner Mongolia Cashmere Goat

Summary

Introduction

Insulin-like growth factor-binding proteins (IGFBPs) are a family of secreted proteins that bind insulin-like growth factors I and II (IGFs I and II). The function of IGFBP-5 is mainly emphasized on cell growth and the protein either suppresses or induces cell proliferation (Andress and Birnbaum, 1992; Ewton et al, 1998; Meadows et al, 2000; Schneider et al, 2001). It interacts with several extracellular matrix proteins including thrombospondin-1 (Nam et al, 2002), osteopontin (Nam et al, 2002), vitronectin (Nam et al, 2000) and plasminogen activator inhibitor-1 (Nam et al, 1997). In the past few years, it has been shown that the effect of IGFBP-5 on cell survival seems to be based on interactions with specific soluble or cell-associated ligands (Beattie et al, 2006; Hung et al, 2008; Schneider et al, 2002)

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