Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)

Mei Jin,Qian Cao,Jun Piao,Fengqin Zhao,Jing’Ai Piao,Ruilong Wang

doi:10.5713/ajas.16.0078

Mei Jin, Qian Cao + Show 4 more

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https://doi.org/10.5713/ajas.16.0078

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Abstract

ObjectiveAn experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.MethodsIn previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization.ResultsBioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb.ConclusionWe conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.

Highlights

Cashmere is a heterogeneous fleece mainly composed of unmedulated cashmere and medulla developed wool, which are, respectively, generated by the secondary and the primary hair follicles [1]
Tissue distribution of the Liaoning cashmere goat KAP11.1 mRNA To determine the steady-state level expression of the KAP11.1 gene in different goat tissues, semi-quantitative RT-PCR analysis was carried out with total RNA extracted from skin, heart, liver, Figure 1
The results of semi-quantitative RT-PCR in adult goat tissues indicated that KAP11.1 mRNA was strongly expressed in skin, heart and liver while its expression was restricted in lung, spleen, and kidney in our study

Summary

Introduction

Cashmere is a heterogeneous fleece mainly composed of unmedulated cashmere and medulla developed wool, which are, respectively, generated by the secondary and the primary hair follicles [1]. Cashmere characteristics are distinctly different depending on the region and goat species [3]. Cashmere is the derivative of the secondary hair follicles [1] and is the inner hair of the goat. Keratins and keratin-associated proteins (KAPs) are the main structural proteins of the hair, which determine the essential characteristic of cashmere and wool [4]. KAPs are structural proteins used for encoding hair fiber. More than 100 KAP genes that have been grouped into 27 KAP families have been identified in mammalian species [6]. In sheep all the KAP genes that have been identified www.ajas.info

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