Molecular characterization and expression of the Prostaglandin reductase 1 gene and protein during ovarian development of the giant tiger shrimp Penaeus monodon

Abstract

Prostaglandin reductase 1 (PTGR1; also called NADP +-dependent leukotriene B4 12-hydroxydehydrogenase, LTB4DH) is the key enzyme responsible for biological inactivation of prostaglandins and related eicosanoids. In this study, the full-length cDNA of PTGR1 in the giant tiger shrimp ( Penaeus monodon) was characterized. PmPTGR1 was 2405 bp in length with an ORF of 1035 bp encoding a polypeptide of 344 amino acids. Interestingly, its 3′ UTR contained the nucleotide sequence (825 bp) that significantly matched positions 3–277 (with 4 amino acid variants) of the deduced P. monodon peritrophin2 protein. PmPTGR1 was more preferentially expressed in ovaries than testes of P. monodon broodstock. In intact broodstock, PmPTGR1 was up-regulated in early cortical rod (stage III) ovaries ( P < 0.05) and comparably expressed afterwards ( P > 0.05). In eyestalk-ablated broodstock, PmPTGR1 was temporally lower in early cortical rod compared to previtellogenic (I) and vitellogenic (II) ovaries ( P < 0.05) and returned to the previous level in mature (IV) ovaries ( P < 0.05). More importantly, the relative expression level of PmPTGR1 in each ovarian stage in eyestalk-ablated females was lower than that in intact P. monodon broodstock ( P < 0.05). This strongly suggested that eyestalk ablation potentially affects the expression of PmPTGR1 allowing the stimulating effects of prostaglandins and related eicosanoids on vitellogenesis and ovarian maturation of P. monodon. The level of ovarian PmPTGR1 protein seemed to increase during ovarian development in intact broodstock but slightly reduced in mature ovaries in eyestalk-ablated broodstock. Results suggested the possible contribution of PmPTGR1 in ovarian development of P. monodon.