Abstract
BackgroundCanker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry. However, resistance proteins in plants play crucial roles to against pathogen infection. Among resistance proteins, the leucine-rich repeat (LRR) protein is a major family that plays crucial roles in plant growth, development, and biotic and abiotic stress responses, especially in disease defense.ResultsIn the present study, a transcriptomics analysis identified a total of 272 LRR genes, 233 of which had coding sequences (CDSs), in the plant pitaya (Hylocereus polyrhizus) in response to fungal Neoscytalidium dimidiatum infection. These genes were divided into various subgroups based on specific domains and phylogenetic analysis. Molecular characterization, functional annotation of proteins, and an expression analysis of the LRR genes were conducted. Additionally, four LRR genes (CL445.Contig4_All, Unigene28_All, CL28.Contig2_All, and Unigene2712_All, which were selected because they had the four longest CDSs were further assessed using quantitative reverse transcription PCR (qRT-PCR) at different fungal infection stages in different pitaya species (Hylocereus polyrhizus and Hylocereus undatus), in different pitaya tissues, and after treatment with salicylic acid (SA), methyl jasmonate (MeJA), and abscisic acid (ABA) hormones. The associated protein functions and roles in signaling pathways were identified.ConclusionsThis study provides a comprehensive overview of the HpLRR family genes at transcriptional level in pitaya in response to N. dimidiatum infection, it will be helpful to understand the molecular mechanism of pitaya canker disease, and lay a strong foundation for further research.
Highlights
Canker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry
Identification and phylogenetic analysis of pitaya HpLRR transcriptional genes We have obtained the differentially expressed Hiseq data induced by pitaya canker disease through RNA-seq reported in detail in our previous study [45]
Heatmaps of the 272 HpLRR gene expression levels in D1, D3, N2 and N3 samples are presented in Figs. 1 and 2
Summary
Canker disease caused by Neoscytalidium dimidiatum is a devastating disease resulting in a major loss to the pitaya industry. Resistance proteins in plants play crucial roles to against pathogen infection. The leucine-rich repeat (LRR) protein is a major family that plays crucial roles in plant growth, development, and biotic and abiotic stress responses, especially in disease defense. Once attacked by a pathogen, plants perceive and recognize the pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) via cell surface receptors and trigger an immune response, which is known as PAMP or MAMP-triggered immunity (PTI/MTI) [1]. Plant first to activate defenses by plant pattern recognition receptors (PRRs) which located in cell surface. Surface-localized receptor kinases (RKs), especially the TM-LRR are the most known PRR proteins. One kind of the RKs is surfacelocalized receptor and the other are receptor-like proteins These proteins can detect conserved PAMPs by various ligand-binding ectodomains to activate plant PTI/MTI [3]. Compared with PRR-RKs, the PRR-RLPs proteins have the same overall structures but lack an intracellular kinase domain
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
