Abstract
Many of the plant leucine rich repeat receptor-like kinases (LRR-RLKs) have been found to regulate signaling during plant defense processes. In this study, we selected and sequenced an LRR-RLK gene, designated as Oryza rufipogon receptor-like protein kinase 1 (OrufRPK1), located within yield QTL yld1.1 from the wild rice Oryza rufipogon (accession IRGC105491). A 2055 bp coding region and two exons were identified. Southern blotting determined OrufRPK1 to be a single copy gene. Sequence comparison with cultivated rice orthologs (OsI219RPK1, OsI9311RPK1 and OsJNipponRPK1, respectively derived from O. sativa ssp. indica cv. MR219, O. sativa ssp. indica cv. 9311 and O. sativa ssp. japonica cv. Nipponbare) revealed the presence of 12 single nucleotide polymorphisms (SNPs) with five non-synonymous substitutions, and 23 insertion/deletion sites. The biological role of the OrufRPK1 as a defense related LRR-RLK is proposed on the basis of cDNA sequence characterization, domain subfamily classification, structural prediction of extra cellular domains, cluster analysis and comparative gene expression.
Highlights
Plant receptor-like kinases (RLKs) form one of the largest gene families and are grouped within the RLK/Pelle family
Plant leucine-rich repeat (LRR)-RLK family members play a vital role in plant defense or developmental related pathways by perceiving extracellular signals such as plant hormones or pathogen-associated molecular patterns (PAMPs) respectively
Full-length cDNAs were isolated for the RPK1 from O. rufipogon
Summary
Plant receptor-like kinases (RLKs) form one of the largest gene families and are grouped within the RLK/Pelle family. A large number of RLK/Pelle family members have been reported for Arabidopsis thaliana (1027) and rice (1429) [1]. The RLK/Pelle family can be sub-classified into more than 40 subfamilies based on their sequences and structural analysis [2,3]. Leucine-rich repeat (LRR)-like transmembrane receptor kinases represent the largest subfamily of the plant RLK/Pelle family. These proteins contain an extracellular domain connected to a cytoplasmic serine/theronine protein kinase domain via a single pass transmembrane helix [3]. Plant LRR-RLK family members play a vital role in plant defense or developmental related pathways by perceiving extracellular signals such as plant hormones or pathogen-associated molecular patterns (PAMPs) respectively
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