Molecular characterization and association with carcass traits of the porcine SLC39A7 gene

Abstract

In this study, the molecular characterization and potential association of SLC39A7 gene with carcass traits were investigated in pigs. The sequence of SLC39A7 cDNA was obtained by in silico cloning and RT-polymerase chain reaction (PCR). Two transcripts, variant 1 (2398 bp) and variant 2 (2088 bp), of the SLC39A7 gene were identified. Expression analysis of SLC39A7 in 10 different tissues by RT-PCR showed that variant 1 was ubiquitously expressed in all tissues analysed, but variant 2 was not found in fat tissue. The cDNA regions of variant 1 and 2 were organized in seven and eight exons respectively. A c.205G>A substitution in exon 3, which changes a codon for glycine into a codon for arginine, (p.Gly69Arg) and a c.1138-216T>C substitution in intron 6 were detected by PCR-HpaII-restriction fragment length polymorphisms (RFLP) and PCR-cofI-RFLP respectively. Significant differences were found in the allele frequencies of c.205G>A among six Chinese indigenous pig breeds and two commercial pig breeds. Linkage analysis showed that the c.205G>A polymorphism within the SLC39A7 gene was closely linked to the marker Sw1856 on pig chromosome 7 in a Large White x Meishan F(2) resource population. The QTL and association studies between polymorphisms of the SLC39A7 gene and carcass traits were carried out. Significant associations of the SLC39A7 polymorphisms with backfat thickness at thorax-waist (p < 0.05), average backfat thickness (p < 0.05) and leaf fat weight (p < 0.01) were found. Additional F-drop test or marker assisted association analyses also supported the association of the mutation in SLC39A7 with the above traits. Together, the present study provided the useful information for the characterization of SLC39A7 gene and potential association with carcass traits in pigs.