Abstract
Morphology-based taxonomy is possible to identify accurately the genus level, but the species level identifications are exactly complicated, subjective, laborious, and lead to erroneous identification/classification. The traditional methods defined the overestimate species, while those in the mycelia appearance or those that have slow growth in culture are mostly overlooked (Davet and Rouxel 1997). The culturable isolates can utilize the energy source under the physical and chemical limitations of the growth medium. The taxonomy classification of Trichoderma has limitations until today. Many Trichoderma species have similar physical appearance under cultural conditions and show similar morphological characters, although they are completely different species. Handling of these cultures is time-consuming and laborious when a large number of isolates are used. Following these practices, the possibility of culture contamination is high, especially the fast-growing fungi always dominating slow-growing fungi inside the medium. So it is recommended that multiplications approaches should be applied for the accurately identification of Trichoderma isolates at the species level.
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