Abstract
Objective To investigate the distribution of integrons and integrated gene cassettes in Enterobacteriaceae isolated from urine and analyze their relations between integrons and drug resistance.Methods One hundred and eleven clinical non-repeated isolates of Enterobacteriaceae from urine samples were selected.Automatic bacterial analysis system was used to identify and test antimicrobial susceptibility of 111 clinical isolates.Class 1,2 and 3 integron integrase genes( int11 、int12、int13) were screened by PCR,the inserted gene cassettes of variable regions in intll positive isolates were detected by using PCR combined with sequence analysis.Results Among 111 Enterobacteriaceae clinical non-duplicate isolates from urine,int11 were detected in 69 isolates(62％ ) and int12 in 12 isolates( 11％ ),no int13 gene was detected.Six kinds of variable fragments of different lengths were detected in int11 positive isolates:800bp fragment did not contain gene cassette,1500 bp fragment containd gene cassette aadB,1800 bp fragment containd gene cassette aadA2,2200 bp fragment was gene cassette array aac(6' )-Ib-cmlA1,3500 bp fragment was aadB-catB3-aadA1 and 3000 bp was dfrA32.dfrA32 was detected for the first time in a Proteus mirabilis as a new kind of trimethoprim resistance gene.The resistance rates of many antimicrobial agents of class 1 integron-positive strains tested in this study were higher than those of the negatives.Conclusions Class 1 integrons as well as their associated gene cassettes are widely distributed among Enterobacteriaceae and closely related to antimicrobial agent resistance. Key words: Integrons; Gene cassette; Drug resistance
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