Abstract
Simple SummaryBactrocera dorsalis fat body protein 1 (Bdfbp1) cDNA was cloned. The deduced amino acid sequence contains three motifs: hemocyanin N, high molecular weight glutenin (gultenin hmw), and hemocyanin C from N to C termini. The glutenin hmw allows Bdfbp1 to fold into a compact form for storage. Bdfbp1 was highly expressed in the late third instar larvae and day 0 pupae. This suggests that Bdfbp1 is stored during larval stages as a storage protein for construction of adult tissues during pupal stages, and may be associated with adult eclosion.Bactrocera dorsails fat body protein 1 (Bdfbp1) cDNA was cloned (GenBank accession no. MT514270), and the complete 3,749-bp cDNA encoded a 1,152-amino acid protein. The phylogenetic relationship of dipteran fbp1s was analyzed. The sequence XP_028900815 from the insect genome project for Zeugodacus cucurbitae (LOC105219342) was proposed that two fbp1 genes were present in the sequence. The developmental transcriptional expression profiles were determined. In the larval stages, Bdfbp1 mRNA had significantly higher expression in the late third instar larvae compared with first, second, and early third instar larvae. In the pupal stages, the highest expression of Bdfbp1 mRNA was found in the newly pupated pupae and then decreased with age. In the fat body of female adults, Bdfbp1 was highly expressed in newly emerged samples and decreased rapidly over the following three days. In the fat body of male adults, Bdfbp1 was highly expressed in newly eclosed samples. RNAi treatment decreased the expression level of Bdfbp1 without statistical difference. However, RNAi treatment significantly decreased the rate of eclosion. These results suggest that Bdfbp1 may function as a storage protein and be associated with adult eclosion.
Highlights
The fat body, an essential tissue for insect metabolism, provides energy and synthesizes proteins for insect development and reproduction
In B. dorsalis, the storage protein, hexamerin gene BdAr is expressed in third instar larvae, pupae, and adults, with especially high expression levels in late third instar larvae and day 0 adults [12]
A 641-bp cDNA fragment encoding partial Bactrocera dorsails fat body protein 1 (Bdfbp1) was obtained from the day 0 (D0) fat body cDNA library [6]
Summary
The fat body, an essential tissue for insect metabolism, provides energy and synthesizes proteins for insect development and reproduction. The oriental fruit fly, Bactrocera dorsalis, is one of the most disastrous pests, leading to damages of about 450 plant species throughout the world [1] Their high fecundity makes them preeminent, with 1000 eggs laid over the entire life-span of females [2]. A comparison of gene expression between the larval- and adult-form fat body in B. dorsalis adults reveals that the arylphorin receptor is expressed more abundantly in the larval form [6]. In B. dorsalis, the storage protein, hexamerin gene BdAr is expressed in third instar larvae, pupae, and adults, with especially high expression levels in late third instar larvae and day 0 adults [12]. Because B. dorsalis fbp (Bdfbp1) is highly expressed in larval-form fat body as BdAr is [6], this makes us to hypothesize that fbp functions as a storage protein. The developmental transcription profiles of Bdfbp were determined through real-time quantitative polymerase chain reaction, and the function of Bdfbp related to adult eclosion was explored through RNA interference
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