Abstract
We report a novel mycovirus with a positive-sense single-stranded (+)ss RNA genome, belonging to the family Hypoviridae, infecting Trichoderma harzianum strain M6. The complete genome sequence is 13,813 nucleotides long, excluding the poly(A) tail at the 3' end. Sequence analysis revealed that the genome has a single large open reading frame (ORF) encoding a 4,118-amino-acid polyprotein harboring five conserved motifs of a protease, two conserved domains of a protein of unknown function, an RNA-dependent RNA polymerase, and a helicase. Sequence comparisons revealed that the deduced amino acid sequence of the polyprotein is similar to those of other hypoviruses and is most similar to that of Bipolaris oryzae hypovirus 1 (35.1% identity). Phylogenetic analysis using full-length RdRp and helicase sequences showed that this virus clustered closely with known members of the proposed genus "Alphahypovirus" of the family Hypoviridae. We accordingly designated this novel mycovirus "Trichoderma harzianum hypovirus 2" (ThHV2).
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