Abstract
Glioblastoma multiforme (GBM) is a clinically and pathologically heterogeneous brain tumor. Previous studies of transcriptional profiling have revealed biologically relevant GBM subtypes associated with specific mutations and dysregulated pathways. Here, we applied a modified proteome to uncover abnormal protein expression profile in a MRI-classified group I GBM (GBM1), which has a spatial relationship with one of the adult neural stem cell niches, subventricular zone (SVZ). Most importantly, we identified molecular characteristics in this type of GBM that include up-regulation of metabolic enzymes, ribosomal proteins, and heat shock proteins. As GBM1 often recurs at great distances from the initial lesion, the rewiring of metabolism, and ribosomal biogenesis may facilitate cancer cells’ growth and survival during tumor progression. The intimate contact between GBM1 and the SVZ raises the possibility that tumor cells in GBM1 may be most related to SVZ cells. In support of this notion, we found that markers representing SVZ cells are highly expressed in GBM1. Emerged findings from our study provide a specific protein expression profile in GBM1 and offer better prediction or therapeutic implication for this multifocal GBM.
Highlights
Glioblastoma multiforme (GBM), a devastating disease with limited therapeutic options, is a highly aggressive brain cancer characterized by uncontrolled proliferation, resistance to cell death, robust angiogenesis, and vascular edema
Proteins that were highly expressed in GBM1 compared to normal brain region were inferred from the confidence, in that top-ranked amino acid sequences could be assigned to MS/MS spectra of tryptic peptides cleaved from top-ranked proteins
Studies depicting the mechanism of glioma formation have been hampered by the fact that GBM is a dynamic disease
Summary
Glioblastoma multiforme (GBM), a devastating disease with limited therapeutic options, is a highly aggressive brain cancer characterized by uncontrolled proliferation, resistance to cell death, robust angiogenesis, and vascular edema. The gene expression signature of GSC resembles those of embryonic stem cells (ESC) and neural stem cells (NSCs), suggesting GSC share features with nonneoplastic stem cells. A recent report suggested that this type of brain tumor could develop through reprograming of mature cells into progenitor-like cells by oncogenic factors [10]. Independent of these hypotheses, a previous study by MRI for the spatial relationship of the contrast enhancing lesion (CEL) with the SVZ and cortex has revealed that group 1 GBM (GBM1) contacts the SVZ intimately and recurs at great distances from the initial lesion [11]. Since the SVZ harbors cells with great proliferative potential and the microenvironment within SVZ is permissive to growth and proliferation, this neurogenic niche is suspected to be a vulnerable site for the origin of subtypes of GBM
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