Abstract

BackgroundColistin has been considered as a last-line treatment option in severe infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, the emergence of the mobile colistin resistance gene (mcr-1) has challenged this viewpoint. The aim of this study is to explore the prevalence of mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and investigate their molecular characteristics.MethodsA total of 700 E. coli isolates were used to screen mcr-1 by PCR and sequencing in a Chinese university hospital from August 2014 to August 2015. Susceptibility test of mcr-1-producing isolates was determined by Vitek -2 Compact system. 26 virulence factors (VFs), phylogenetic groups, Multi-locus sequence typing (MLST), and DNA Fingerprinting (ERIC-PCR) of strains were investigated by PCR.ResultsFour (0.6%) mcr-1 producing E. coli isolates were found in this study. The results of antibiotic susceptibility test showed that all four isolates were resistant to colistin, ciprofloxacin, levofloxacin, cefazolin, and trimethoprim/sulfamethoxazole, and were susceptible to amikacin, ertapenem and imipenem. In addition, all 4 isolates exhibited high-level resistance to aztreonam, cefotaxime and gentamicin. The numbers of VFs contained in mcr-1 positive isolates were no more than 4 in our study. MLST result demonstrated that these isolates were assigned to two sequence types: ST156 and ST167. The result of phylogenetic analysis showed that four mcr-1-positive isolates belong to two phylogenetic groups: A and B1 group. ERIC-PCR showed that four mcr-1 positive strains were categorized into three different genotypes.ConclusionsOur study demonstrated a low prevalence of mcr-1 in E. coli clinical isolates in a Chinese teaching hospital, and we have gained insights into the molecular characteristics of these mcr-1-positive strains. Increasing the surveillance of these infections, as well as taking effective infection control measures are urgently needed to take to control the transmission of mcr-1 gene.

Highlights

  • Colistin has been considered as a last-line treatment option in severe infections caused by multidrugresistant (MDR) gram-negative pathogens

  • Mcr-1 was firstly found in Escherichia coli (E. coli), and it has been spreading to other Enterobacteriaceae [9]

  • The aim of this study was to evaluate the prevalence of mcr-1 in E. coli clinical isolates in a Chinese teaching hospital, and to investigate the molecular characteristics of these strains

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Summary

Introduction

Colistin has been considered as a last-line treatment option in severe infections caused by multidrugresistant (MDR) gram-negative pathogens. The emergence of the mobile colistin resistance gene (mcr-1) has challenged this viewpoint. The aim of this study is to explore the prevalence of mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and investigate their molecular characteristics. The prevalence of the mobile colistin resistance gene (mcr-1) in animals and human beings worldwide. Since it was initially found, plasmid-mediated mcr-1 has been detected widely [3, 7]. Several reports showed that the mcr-1 gene could coexist with other resistance genes The aim of this study was to evaluate the prevalence of mcr-1 in E. coli clinical isolates in a Chinese teaching hospital, and to investigate the molecular characteristics of these strains

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