Abstract
The effect of different dissolution procedures on molecular characteristics of waxy starches from corn and barley were evaluated using a MALLS (batch-mode) detector, principally to understand the role played by different solvents. Starches were dissolved in aqueous solutions of: (1) 90% DMSO, (2) 2M KOH and (3) 90% DMSO/2M KOH, and stored for 15days at ∼22°C with shaking. Weight-average molecular weight (Mw) and z-average radius of gyration (Rz) were determined at intervals ranging from 0 to 15days. Regardless of starch source and dissolution procedures, Mw and Rz of amylopectin (AP) decreased with increasing storage time. Decrease in Mw followed the order: 90% DMSO>2M KOH>90% DMSO/2M KOH. However, decrease in Rz followed the order: 2M KOH>90% DMSO/2M KOH>90% DMSO. The study indicated that starch solubilisation in 90% DMSO/2M KOH may be a reasonable method for molecular characterisation with less AP degradation.
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