Abstract
Type II collagen (CII) was purified from chick sternal cartilage using a combination of pepsin digestion, NaCl precipitation and ion exchange chromatography. Following, the effect of purified CII on the collagen-induced rat arthritis (CIA) model was investigated. Circular dichroism spectral and atomic force microscopy analysis indicated that the purified CII retained its intermolecular cross-links during the preparation process. Compared with the control group, oral administration of 600 µg/kg CII over a period of 35 days markedly decreased the index of arthritis (30.98%) and suppressed paw swelling (20.28%) in CIA rats. Furthermore, CII treatment also dose-dependently reduced the serum level of anti-CII antibody and inhibited the over-production of inflammatory cytokines levels (tumour necrosis factor α, interleukin 1 β and interferon γ) in CIA rats. In conclusion, CII extracted from chick sternal cartilage possesses anti-rheumatoid arthritis activity, which may be a result of its regulation of the humoral and cellular immune systems.
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