Molecular characterisation of tomato leaf miner Tuta absoluta populations obtained from different geographical locations of India

Abstract

The tomato leaf miner, Tuta absoluta (Meyrick) is one of the major invasive insect pests on solanaceous crops. T. absoluta distribution is observed in European, North African Mediterranean basin and Asian countries. The tomato leaf miner is spreading fast and affecting tomato production in both closed and open-field conditions. This insect pest has recently reported in various tomato growing regions of India and causing significant loss of production. In the present study, occurrence of a tomato leaf miner was studied by undertaking an extensive survey in tomato growing regions of India during September 2017 to May 2018. The infestation of T. absoluta was recorded in Maharashtra, Karnataka, Telangana, Tamil Nadu, Haryana and Himachal Pradesh. Severe incidence of T. absoluta infestation was recorded in Maharashtra (Nashik district) and Telangana (Mahabubnagar district) followed by Karnataka (Kalaburgi and Raichur districts). The moths collected during the survey were characterized using mitochondrial cytochrome c oxidase subunit I (COI) gene analysis. The T. absoluta samples showed maximum genetic similarity with KU565720 Kenya, KT452897 Oman, KY212128 South Africa, KY619687 India, KP814057 India and KJ657679 Florida. Nine T. absoluta populations were grouped under a single clade revealing no genetic variation within populations and showed high genetic homogeneity thereby the ideal candidate for sterile insect technique application. Further studies are required on population dynamics, host range, local and area-wide biological control management strategies for the effective management of T. absoluta .