Abstract
The fowl adenovirus field strain FAdV-JSN-5/10j (GenBank accession number KP879219) was isolated from the intestine of a 7-week-old chicken diagnosed with inclusion body hepatitis and simultaneously with Marek’s disease, and for that reason, it was chosen for molecular study. It was identified as fowl adenovirus genotype 7 (species Fowl aviadenovirus E) based on nucleotide sequence analysis of the loop L1 region of the hexon gene. Nucleotide sequence alignment of this strain, FAdV-7 reference strains B-3A ATCC VR-832 (AF339922) and YR36 (AF508955), and eight additional FAdV-7 field strains confirmed its classification as FAdV-JS-5/10j and showed that these viruses are very similar to each other. Additionally, we described mutations and their influence on the amino acid sequence, nucleotide composition, and relative synonymous codon usage. Immunofluorescence of cell cultures infected with 104.5 TCID 50 per 0.1-ml dose of the FAdV-JSN-5/10j strain demonstrated the presence of a cytopathic effect. Infection of fowl with adenoviruses raises concerns for poultry production, and thus, the efficient detection of adenovirus infection is crucial. This is the first attempt to describe the molecular characteristics of FadV-7 strains isolated in Poland.
Highlights
Viruses belonging to the family Adenoviridae are divided into five genera
The aim of this study was to determine the nucleotide sequence of a portion of the genome of a field isolate from a sick chicken and to determine the relative synonymous codon usage (RSCU) in the loop L1 region of the hexon gene, as well as to compare it with two sequences of reference strains and field strains obtained from the GenBank database
Strain JSN-5/10j caused cytopathic effect (CPE), which was compared to the CPE obtained with ATCC Fowl adenoviruses (FAdVs)-7, which was used as a control
Summary
Viruses belonging to the family Adenoviridae are divided into five genera. Fowl adenoviruses (FAdVs) belong to the genus Aviadenovirus, which is further divided into 12 species: Fowl aviadenovirus A-E, Duck aviadenovirus B, Falcon aviadenovirus A, Goose aviadenovirus A, Pigeon aviadenovirus A, and Turkey aviadenovirus B-D. FAdVs are divided into twelve serotypes (1-8a-8b-11). A significant percentage of the different FAdV serotypes cause disease in poultry [1, 8]. Avian adenovirus serotypes 6, 7 and 8 have been reported to cause inclusion body hepatitis (IBH) in Australia, New Zealand, and other parts of the world [20]. FAdVs were detected in liver samples obtained from broiler chicken flocks with IBH or hydro-pericardium syndrome (HPS) by PCR using the hexon gene [22]. In Pakistan, many FAdV strains of serotype FAdV-4 have been isolated from broiler flocks with HPS [27]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
