Abstract
Salmonella multidrug-resistant clinical organisms identified as serotype [4,5,12:i:−] were typed using selected genetic procedures and compared with typhimurium organisms collected in the same Spanish region. Results showed a low genetic heterogeneity among [4,5,12:i:−] organisms, which generated identical ribotypes and similar but not identical XbaI PFGE, RAPD, and plasmid profiles. Multidrug resistance could be eliminated by curing and seems to be mediated by 140-kb ( spvC+) and 120-kb ( spvC−) non-self-transferable plasmids. The [4,5,12:i:−] organisms fall into a single genetic lineage, which emerged in 1997 and presents a different degree of genetic relationship with typhimurium lineages.
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