Abstract
The objectives of the present study were to characterise Aeromonas hydrophila isolated from diseased tilapia Oreochromis niloticus cultured in Kafrelsheikh farms and to evaluate virulence and pathogenecity. About 200 adult fish exhibiting the onset of clinical signs were bacteriologically examined using Aeromonas isolation medium. The isolates were identified by 16S rRNA gene sequence analysis using species specific primers and then tested for ten virulence genes employing specific primers. Experimental challenge studies were also conducted to investigate the pathogenicity of the recovered A. hydrophilastrains. Thirty five isolates were identified as A. hydrophila based on 16S rRNA gene sequence analysis,which were also positive for presence of four virulence genes (aerolysin, lipase, cytotonic heat stable enterotoxin and haemolysin). Results showed that majority of the examined strains carried one or more virulence genes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.