Molecular changes in the sarcoplasmic reticulum calcium ATPase during catalytic activity: A Fourier transform infrared (FTIR) study using photolysis of caged ATP to trigger the reaction cycle

Abstract

Fourier transform infrared spectroscopy was used to study ligand binding and conformational changes in the Ca 2+-ATPase of sarcoplasmic reticulum. Novel in infrared difference spectroscopy, the catalytic cycle in the IR sample was started by photolytic release of ATP from an inactive, photolabile ATP-derivative (caged ATP). Small, but characteristic infrared absorbance changes were observed upon ATP release. On the basis of model spectra, the absorbance changes corresponding to the trigger and substrate reactions, i.e. to photolysis of caged ATP and hydrolysis of ATP, were separated from the absorbance changes due to the active ATPase reflecting formation of the phosphorylated Ca 2E 1P enzyme form. A major rearrangement of ATPase conformation as the result of catalysis can be excluded.