Molecular Breeding for Improving Productivity of Oryza sativa L. cv. Pusa 44 under Reproductive Stage Drought Stress through Introgression of a Major QTL, qDTY12.1.

Kyaw Swar Oo,Priyanka Dwivedi,Viswanathan Chinnuswamy,Kunnummal Kurungara Vinod,Mariappan Nagarajan,Pankaj Kumar,Prolay Kumar Bhowmick,Gaurav Dhawan,Subbaiyan Gopala Krishnan,Ashok Kumar Singh,Haritha Bollinedi,Ranjith Kumar Ellur,Madan Pal

doi:10.3390/genes12070967

Kyaw Swar Oo, Priyanka Dwivedi + Show 11 more

Open Access

https://doi.org/10.3390/genes12070967

Copy DOI

Journal: Genes	Publication Date: Jun 24, 2021
Citations: 6	License type: CC BY 4.0

Affiliation: Indian Agricultural Research Institute

Abstract

Increasing rice production is quintessential to the task of sustaining global food security, as a majority of the global population is dependent on rice as its staple dietary cereal. Among the various constraints affecting rice production, reproductive stage drought stress (RSDS) is a major challenge, due to its direct impact on grain yield. Several quantitative trait loci (QTLs) conferring RSDS tolerance have been identified in rice, and qDTY12.1 is one of the major QTLs reported. We report the successful introgression of qDTY12.1 into Pusa 44, a drought sensitive mega rice variety of the northwestern Indian plains. Marker-assisted backcross breeding (MABB) was adopted to transfer qDTY12.1 into Pusa 44 in three backcrosses followed by four generations of pedigree selection, leading to development of improved near isogenic lines (NILs). Having a recurrent parent genome (RPG) recovery ranging from 94.7–98.7%, the improved NILs performed 6.5 times better than Pusa 44 under RSDS, coupled with high yield under normal irrigated conditions. The MABB program has been modified so as to defer background selection until BC3F4 to accelerate generational advancements. Deploying phenotypic selection alone in the early backcross generations could help in the successful recovery of RPG. In addition, the grain quality could be recovered in the improved NILs, leading to superior selections. Owing to their improved adaptation to drought, the release of improved NILs for regions prone to intermittent drought can help enhance rice productivity and production.

Highlights

Rice is grown in more than 100 countries across the world on a combined total of162.06 million hectares [1]
We report the successful introgression of qDTY12.1 into Pusa 44 thrcoruogphprmodarukcetiro-ansasnisdteednsbuarcikncgrfoososdbsreeceudriintyg,ulnedaedrinchgatnogitnhge cdliemvaeltoe.pMmoenretoovferi,macpcreolevreadted NIdLesvsehloowpminegnitmopf rcouvletidvaardsaupstiantigonthteomRaSrDkSeru-ansdseisrtetwdobrceoendtrinasgtisntgraetengvyirwonomuledntbseina Ibno-on diao.ver classical approaches, which normally take 10–12 years for cultivar development
As hypothesized in this study, future rice cultivars should possess multiple stress tolerance combined with higher yield and desirable grain quality

Summary

Introduction

Rice is grown in more than 100 countries across the world on a combined total of162.06 million hectares [1]. Grown either under irrigated or rainfed conditions, rice suffers yield loss up to 65.0% under rainfed upland and rainfed lowland ecosystems due to the intermittent occurrence seasonal drought stress. Drought stress during the vegetative growth stage, causes plant height reduction with less effective number of tillers per hill, reduced leaf area, and general stunting [4]. The selections from the BC3F4 lines after agronomic and grain quality evaluation were sent to RBGRC, ICAR-IARI, Aduthurai, where the lines were grown under irrigated conditions. Selections from this trial (Supplementary Table S1) were evaluated at ICAR-IARI, New Delhi during

Methods

Results

Conclusion