Abstract
Immunological cross-reactions were found between each of the four major virus-specific polypeptides of Marek's disease virus (MDV) strains CVI 988, K and HPRS-16/att, and herpesvirus of turkey (HVT) Fc126 by one-dimensional (1D) gel analysis of immunoprecipitates from the lysate or culture medium of infected cells. The results, however, did not allow a serotype classification of MDV and HVT strains. Comparison of the two-dimensional (2D) gel patterns of virus-specific polypeptides of nine MDV and HVT strains with different biological properties revealed many similarities. Strain CVI 988 provided the reference pattern, consisting of 35 polypeptides, 18 of which were glycosylated. Based on their similarities in migration characteristics (size, charge, and heterogeneity of spots) during 2D gel electrophoresis, 11 virus-specific polypeptides or polypeptide complexes were identified in the patterns of nearly all virus strains analyzed. One of these polypeptides was glycoprotein gp5, the putative A antigen of MDV and HVT, which was also detected in the medium of cells infected with HPRS-16/att, a strain which was reported to have lost its capacity for production of A antigen. In addition to the similarities mentioned above, differences were found in migration behavior of virus-specific polypeptides (complexes) p4/p5/p6, gp3, gp5, and gp8/gp9, which were confirmed by coelectrophoresis experiments. The most conspicuous difference was found between three patterns of gp5 which seem to be characteristic for three groups of viruses: (I) high- and low-virulent MDV strains and their attenuated variants; (II) avirulent and apparently nononcogenic MDV strains; (III) herpesviruses of turkey. The minor differences found for the other polypeptides mentioned above further substantiated this molecular-biological classification of MDV and HVT strains and, in addition, enabled the differentiation of two HVT strains within molecular-biological group III.
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